Albion's under-23 team will play the first test event at Tottenham's new stadium next month.
The Premier League 2 fixture on August 27 will be used by the north London club to achieve a safety certificate for the new stadium.
The game comes seven days after Brighton open their under-23 league campaign away to Arsenal at the Emirates Stadium.
Tottenham supporters will be able to apply to attend the test event - and also a Spurs Legends against Bayern Munich Legends came on September 1 - through two ballots and further details will follow in due course. Priority for both events will be given to Spurs season ticket holders and executive members.